Posttranslational modification (PTM) is the chemical modification of a protein after its translation. It is one of the later steps in protein biosynthesis, and thus gene expression, for many proteins.

The bottom of this diagram shows the modification of primary structure of insulin, as described.

Also, enzymes may remove amino acids from the amino end of the protein, or cut the peptide chain in the middle. For instance, the peptide hormone insulin is cut twice after disulfide bonds are formed, and a propeptide is removed from the middle of the chain; the resulting protein consists of two polypeptide chains connected by disulfide bonds. Also, most nascent polypeptides start with the amino acid methionine because the "start" codon on mRNA also codes for this amino acid. This amino acid is usually taken off during post-translational modification.

Other modifications, like phosphorylation, are part of common mechanisms for controlling the behavior of a protein, for instance activating or inactivating an enzyme.

Post-translational modification of proteins is detected by mass spectrometry or Eastern blotting.

PTMs involving addition of functional groups

The genetic code diagram^[1] showing the amino acid residues as target of modification.

PTMs involving addition by an enzyme in vivo

PTMs involving addition of hydrophobic groups for membrane localization

• myristoylation, attachment of myristate, a C₁₄ saturated acid
• palmitoylation, attachment of palmitate, a C₁₆ saturated acid
• isoprenylation or prenylation, the addition of an isoprenoid group (e.g. farnesol and geranylgeraniol)
  • farnesylation
  • geranylgeranylation
• glypiation, glycosylphosphatidylinositol (GPI) anchor formation via an amide bond to C-terminal tail

PTMs involving addition of cofactors for enhanced enzymatic activity

• lipoylation, attachment of a lipoate (C₈) functional group
• flavin moiety (FMN or FAD) may be covalently attached
• heme C attachment via thioether bonds with cysteins
• phosphopantetheinylation, the addition of a 4'-phosphopantetheinyl moiety from coenzyme A, as in fatty acid, polyketide, non-ribosomal peptide and leucine biosynthesis
• retinylidene Schiff base formation

PTMs involving unique modifications of translation factors

• diphthamide formation (on a histidine found in eEF2)
• ethanolamine phosphoglycerol attachment (on glutamte found in eEF1α)^[2]
• hypusine formation (on conserved lysine of eIF5A (eukaryotic) and aIF5A (archeal))

PTMs involving addition of smaller chemical groups

• acylation, e.g. O-acylation (esters), N-acylation (amides), S-acylation (thioesters)
  • acetylation, the addition of an acetyl group, either at the N-terminus ^[3] of the protein or at lysine residues.^[4] See also histone acetylation.^[5][6] The reverse is called deacetylation.
  • formylation
• alkylation, the addition of an alkyl group, e.g. methyl, ethyl
  • methylation the addition of a methyl group, usually at lysine or arginine residues. The reverse is called demethylation.
• amide bond formation
  • amidation at C-terminus
  • amino acid addition
    • arginylation, a tRNA-mediation addition
    • polyglutamylation, covalent linkage of glutamic acid residues to the N-terminus of tubulin and some other proteins.^[7] (See tubulin polyglutamylase)
    • polyglycylation, covalent linkage of one to more than 40 glycine residues to the tubulin C-terminal tail
• butyrylation
• gamma-carboxylation dependent on Vitamin K^[8]
• glycosylation, the addition of a glycosyl group to either arginine, asparagine, cysteine, hydroxylysine, serine, threonine, tyrosine, or tryptophan resulting in a glycoprotein. Distinct from glycation, which is regarded as a nonenzymatic attachment of sugars.
  • polysialylation, addition of polysialic acid, PSA, to NCAM
• malonylation
• hydroxylation
• iodination (e.g. of thyroglobulin)
• nucleotide addition such as ADP-ribosylation
• oxidation
• phosphate ester (O-linked) or phosphoramidate (N-linked) formation
  • phosphorylation, the addition of a phosphate group, usually to serine, threonine, and tyrosine (O-linked), or histidine (N-linked)
  • adenylylation, the addition of an adenylyl moiety, usually to tyrosine (O-linked), or histidine and lysine (N-linked)
• propionylation
• pyroglutamate formation
• S-glutathionylation
• S-nitrosylation
• succinylation addition of a succinyl group to lysine
• sulfation, the addition of a sulfate group to a tyrosine.
• selenoylation (co-translational incorporation of selenium in selenoproteins)

PTMs involving non-enzymatic additions in vivo

• glycation, the addition of a sugar molecule to a protein without the controlling action of an enzyme.

PTMs involving non-enzymatic additions in vitro

• biotinylation, acylation of conserved lysine residues with a biotin appendage
• pegylation

PTMs involving addition of other proteins or peptides

• ISGylation, the covalent linkage to the ISG15 protein (Interferon-Stimulated Gene 15)^[9]
• SUMOylation, the covalent linkage to the SUMO protein (Small Ubiquitin-related MOdifier)^[10]
• ubiquitination, the covalent linkage to the protein ubiquitin.
• Neddylation, the covalent linkage to Nedd
• Pupylation, the covalent linkage to the Prokaryotic ubiquitin-like protein

PTMs involving changing the chemical nature of amino acids

• citrullination, or deimination, the conversion of arginine to citrulline
• deamidation, the conversion of glutamine to glutamic acid or asparagine to aspartic acid
• eliminylation, the conversion to an alkene by beta-elimination of phosphothreonine and phosphoserine, or dehydration of threonine and serine, as well as by decarboxylation of cysteine ^[11]
• carbamylation, the conversion of lysine to homocitrulline ^[12]

PTMs involving structural changes

• disulfide bridges, the covalent linkage of two cysteine amino acids
• proteolytic cleavage, cleavage of a protein at a peptide bond
• racemization of proline by prolyl isomerase

Post-translational modification statistics

Recently statistics of each post-translational modification experimentally and putatively detected have been compiled using proteome-wide information from the Swiss-Prot database.^[13] These statistics can be found at http://selene.princeton.edu/PTMCuration/.

Case examples

• Cleavage and formation of disulfide bridges during the production of insulin
• PTM of histones as regulation of transcription: RNA polymerase control by chromatin structure
• PTM of RNA polymerase II as regulation of transcription
• Cleavage of polypeptide chains as crucial for lectin specificity

External links

• List of posttranslational modifications in ExPASy
• Statistics of each post-translational modification from the Swiss-Prot database
• AutoMotif Server - A Computational Protocol for Identification of Post-Translational Modifications in Protein Sequences
• Functional analyses for site-specific phosphorylation of a target protein in cells
• Detection of Post-Translational Modifications after high-accuracy MSMS

References

ca:Modificaci postraduccional cs:Posttransla n modifikace da:Posttranslationel modifikation de:Posttranslationale Modifikation es:Modificaci n postraduccional fa: fr:Modification post-traductionnelle it:Modificazione post traduzionale nl:Posttranslationele modificatie ja: pl:Modyfikacje posttranslacyjne pt:Modifica o p s-traducional ru: sr:Posttranslaciona modifikacija tr: evrim sonras de i im uk: zh: